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Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells

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Book cover Dynamin Superfamily GTPases

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2159))

Abstract

Dynamin-like protein 1 (Drp1) is the master regulator of mitochondrial fission. Drp1 translocates from the cytosol to the mitochondrial outer membrane to execute the scission process. Here we describe an immunofluorescence-based method to measure the mitochondrial translocation of Drp1 and quantify Drp1-related mitochondrial fission by labeling the mitochondrial import receptor subunit TOM20 in fixed cell culture.

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References

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Authors and Affiliations

  1. Departments of Physiology & Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH, USA

    Di Hu & Xin Qi

  2. Center for Mitochondrial Diseases, Case Western Reserve University School of Medicine, Cleveland, OH, USA

    Xin Qi

Authors
  1. Di Hu
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  2. Xin Qi
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Corresponding author

Correspondence to Xin Qi .

Editor information

Editors and Affiliations

  1. School of Medicine, Case Western Reserve University, Cleveland, OH, USA

    Rajesh Ramachandran

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Hu, D., Qi, X. (2020). Quantifying Drp1-Mediated Mitochondrial Fission by Immunostaining in Fixed Cells. In: Ramachandran, R. (eds) Dynamin Superfamily GTPases. Methods in Molecular Biology, vol 2159. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0676-6_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0676-6_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0675-9

  • Online ISBN: 978-1-0716-0676-6

  • eBook Packages: Springer Protocols

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