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Pollen and Pollen Tube Biology pp 191–200Cite as

Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2160))

Abstract

Successful fertilization and seed set require the pollen tube to grow through several tissues, to change its growth orientation by responding to directional cues, and to ultimately reach the embryo sac and deliver the paternal genetic material. The ability to respond to external directional cues is, therefore, a pivotal feature of pollen tube behavior. In order to study the regulatory mechanisms controlling and mediating pollen tube tropic growth, a robust and reproducible method for the induction of growth reorientation in vitro is required. Here we describe a galvanotropic chamber designed to expose growing pollen tubes to precisely calibrated directional cues triggering reorientation while simultaneously tracking subcellular processes using live cell imaging and confocal laser scanning microscopy.

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Author information

Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of Minnesota, Saint Paul, MN, USA

    Firas Bou Daher

  2. Department of Plant Science, McGill University, Montreal, QC, Canada

    Anja Geitmann

Authors
  1. Firas Bou Daher
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  2. Anja Geitmann
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Corresponding author

Correspondence to Anja Geitmann .

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Editors and Affiliations

  1. Department of Plant Science, McGill University, Montreal, QC, Canada

    Anja Geitmann

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Bou Daher, F., Geitmann, A. (2020). Galvanotropic Chamber for Controlled Reorientation of Pollen Tube Growth and Simultaneous Confocal Imaging of Intracellular Dynamics. In: Geitmann, A. (eds) Pollen and Pollen Tube Biology. Methods in Molecular Biology, vol 2160. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0672-8_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0672-8_13

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0671-1

  • Online ISBN: 978-1-0716-0672-8

  • eBook Packages: Springer Protocols

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