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Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2156))

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Abstract

A cryo-scanning electron microscope (cryo-SEM) is a valuable tool for observing bulk frozen samples to monitor freezing responses of plant tissues and cells. Here, the essential processes of a cryo-SEM to observe freezing behaviors of plant tissue cells are described.

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Acknowledgments

One of the authors (S.F.) sincerely appreciates the strong support by JEOL Co. Ltd. for improvement and development of a cryo-SEM since he started his studies using a cryo-SEM in 1974. The authors also appreciate the excellent works by Mr. K. Shinbori, Institute of Low Temperature Science, Hokkaido University, for making many apparatuses of a cryo-SEM.

Author information

Authors and Affiliations

  1. Faculty of Agriculture, Hokkaido University, Sapporo, Japan

    Seizo Fujikawa

  2. Forestry and Forest Products Research Institute, Forest Tree Breeding Center, Hitachi, Japan

    Keita Endoh

Authors
  1. Seizo Fujikawa
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  2. Keita Endoh
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Corresponding author

Correspondence to Seizo Fujikawa .

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha

  2. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Ellen Zuther

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Fujikawa, S., Endoh, K. (2020). Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 2156. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0660-5_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0660-5_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0659-9

  • Online ISBN: 978-1-0716-0660-5

  • eBook Packages: Springer Protocols

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