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Exploring Membrane Lipid and Protein Diffusion by FRAP

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Book cover Analysis of Membrane Lipids

Part of the book series: Springer Protocols Handbooks ((SPH))

Abstract

Knowledge of membrane dynamics is crucial since it allows us to understand membrane function. Fluorescence recovery after photobleaching (FRAP) is a widely used technique to monitor diffusion of lipids and proteins in biological membranes. We outline here general aspects of FRAP, followed by a step-by-step guide to carry out FRAP measurements for exploring diffusion of fluorescently labeled lipids and proteins in membranes of attached cells and membranes of Candida albicans. In this process, we have provided detailed hands-on tips, judicious use of which would ensure reliability and quality of acquired FRAP data and associated analysis.

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Acknowledgments

A.C. gratefully acknowledges support from SERB Distinguished Fellowship (Department of Science and Technology, Govt. of India). P.S. thanks the Council of Scientific and Industrial Research for the award of a Shyama Prasad Mukherjee Fellowship. A.C. is a Distinguished Visiting Professor at the Indian Institute of Technology Bombay (Mumbai), Adjunct Professor at the RMIT University (Melbourne, Australia), Tata Institute of Fundamental Research (Mumbai), and Indian Institute of Science Education and Research (Kolkata), and an Honorary Professor at the Jawaharlal Nehru Centre for Advanced Scientific Research (Bengaluru). We thank members of the Chattopadhyay laboratory for their comments and discussions.

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Authors and Affiliations

  1. CSIR-Centre for Cellular and Molecular Biology, Hyderabad, India

    Parijat Sarkar & Amitabha Chattopadhyay

Authors
  1. Parijat Sarkar
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  2. Amitabha Chattopadhyay
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Correspondence to Amitabha Chattopadhyay .

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Editors and Affiliations

  1. Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Gurgaon, Haryana, India

    Rajendra Prasad

  2. Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, India

    Ashutosh Singh

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Sarkar, P., Chattopadhyay, A. (2020). Exploring Membrane Lipid and Protein Diffusion by FRAP. In: Prasad, R., Singh, A. (eds) Analysis of Membrane Lipids. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0631-5_8

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  • DOI: https://doi.org/10.1007/978-1-0716-0631-5_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-0716-0630-8

  • Online ISBN: 978-1-0716-0631-5

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