Abstract
Sphingolipids are a major class of bioactive structural lipids that also play diverse roles in signaling events in different disease conditions like cancer. They form a dynamic metabolic network, and alterations in their metabolism are known to contribute to progression of the disease. Therefore, quantitation of these metabolites from mammalian cells as a disease model system can provide us a vivid picture of the regulatory nodes in the pathway that can be targeted to identify sphingolipid-based biomarkers or targets for future therapies. Herein, we present a simple, sensitive, and robust method to simultaneously quantitate sphingolipids of the early steps of the metabolic pathway by a liquid chromatography–mass spectrometry-based method using mammalian cells as model system.
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Acknowledgments
The support from Amity University Haryana, India is acknowledged. U.D. is supported by BT/PR19624/BIC/101/488/2016 (DBT) and ECR/2016/001603 (DST). Amity Lipidomics Research Facility at Amity University Haryana is supported by DST-FIST grant SR/FST/LSI-664/2016.
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Medatwal, N., Dasgupta, U. (2020). Quantitation of Sphingolipids in Mammalian Cell Lines by Liquid Chromatography–Mass Spectrometry. In: Prasad, R., Singh, A. (eds) Analysis of Membrane Lipids. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0631-5_7
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DOI: https://doi.org/10.1007/978-1-0716-0631-5_7
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