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Insights into Yeast Phospholipid Tra(ffi)cking

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Analysis of Membrane Lipids

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Abstract

Lipids are the best known form of energy storage. Apart from being energy reserves and signaling molecules, they also form an integral part of the cell membranes and provide structure and fluidity to the membranes. A group of polar lipids, namely, the phospholipids (PLs), are membrane constituents, defining the structure, shape, and function of the cells. They decide the cell permeability due to their hydrophobicity. PLs also play an under-rated role in many human diseases, due to which more importance needs to be given toward studies focusing on their synthesis, function, and metabolism. The budding yeast, Saccharomyces cerevisiae, is a model organism for the study of PLs as it is a simple system to characterize lipid metabolic changes under various physiological conditions. Yeasts have been used to study the mechanisms related to lipid metabolism, lipid trafficking, and localization in different subcellular organelles. It also shows the presence of various PLs, which makes it a versatile tool for research. With the recent developments in detection and quantification of PLs, and techniques using novel reagents, tags, and specific stains to localize a particular lipid in different subcellular compartments, yeast makes itself a remarkable model for lipid research. In this chapter, we discuss the various PLs present in the budding yeast, their traffic within the yeast cell, and methods of tracking them.

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Authors and Affiliations

  1. CSIR-Central Food Technological Research Institute, Mysore, India

    Malathi Srinivasan

  2. Central University of Tamil Nadu, Thiruvarur, India

    Ram Rajasekharan

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  1. Malathi Srinivasan
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  2. Ram Rajasekharan
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Correspondence to Malathi Srinivasan .

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Editors and Affiliations

  1. Amity Institute of Biotechnology and Amity Institute of Integrative Sciences and Health, Amity University Haryana, Gurgaon, Haryana, India

    Rajendra Prasad

  2. Department of Biochemistry, University of Lucknow, Lucknow, Uttar Pradesh, India

    Ashutosh Singh

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Srinivasan, M., Rajasekharan, R. (2020). Insights into Yeast Phospholipid Tra(ffi)cking. In: Prasad, R., Singh, A. (eds) Analysis of Membrane Lipids. Springer Protocols Handbooks. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-0631-5_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0631-5_4

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