CRISPR-Cas-Mediated Single Base Editing at More than One Locus in Rice Genome

Part of the Springer Protocols Handbooks book series (SPH)


Base editing is a recently developed technology that enables us to install point mutation in the genome precisely. The fusion of nucleotide deaminase with Cas9 nickase gives rise to base editors. Like the conventional CRISPR-Cas9 technology, a single-guide RNA can direct a base editor to a particular locus to change one nucleotide to another. In this chapter, we describe a stepwise protocol to perform targeted base editing experiments in rice plants.

Key words

CRISPR-Cas9 Base editing Point mutation Rice genome editing Adenine base editors Crop improvement 



Adenine base editor


Base editor


Cytosine base editor


Aspartic acid 10 alanine


Homology-directed repair


Nickase Cas9


Nonhomologous end joining


Protospacer adjacent motif


tRNA adenosine deaminase


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Crop Improvement DivisionNational Rice Research InstituteCuttackIndia
  2. 2.Department of Plant Pathology and Environmental MicrobiologyThe Huck Institute of the Life Sciences, The Pennsylvania State UniversityUniversity ParkUSA

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