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Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization

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Arbuscular Mycorrhizal Fungi

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2146))

Abstract

Histochemical staining and light microscopy-based techniques have been widely used to detect and quantify arbuscular mycorrhizal fungi (AMF) in roots. Here we describe a standardized method for staining of AMF in colonized roots, and we provide possible modifications to adjust the protocol according to particular requirements, such as the type of root material or the reduction of toxic products. In addition, we also summarize some of the most common ways to quantify arbuscular mycorrhizal colonization.

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Acknowledgments

This work was supported by grants from the Comisión Interministerial de Ciencia y Tecnología (CICYT) and Fondos Europeos de Desarrollo Regional (FEDER) through the Ministerio de Economía y Competitividad in Spain (AGL2017-83871-P).

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Authors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín (EEZ) CSIC, Granada, Spain

    Tania Ho-Plágaro, María Isabel Tamayo-Navarrete & José Manuel García-Garrido

Authors
  1. Tania Ho-Plágaro
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  2. María Isabel Tamayo-Navarrete
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  3. José Manuel García-Garrido
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Corresponding author

Correspondence to José Manuel García-Garrido .

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Editors and Affiliations

  1. Department of Soil Microbiology and Symbiotic Systems, Estación Experimental del Zaidín, CSIC, Granada, Spain

    Nuria Ferrol

  2. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università di Torino, Torino, Italy

    Luisa Lanfranco

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Ho-Plágaro, T., Tamayo-Navarrete, M.I., García-Garrido, J.M. (2020). Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization. In: Ferrol, N., Lanfranco, L. (eds) Arbuscular Mycorrhizal Fungi. Methods in Molecular Biology, vol 2146. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0603-2_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0603-2_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0602-5

  • Online ISBN: 978-1-0716-0603-2

  • eBook Packages: Springer Protocols

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