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Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant

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Corneal Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2145))

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Abstract

Cultured limbal epithelial stem cell transplantation is a clinical procedure used to regenerate the corneal epithelium in patients with limbal stem cell deficiency. The protocols used to expand limbal epithelial cells in vitro need to be optimized, since the scarcity of human ocular tissue donors is limiting the potential use of this procedure. Here, we describe a method to consecutively expand a single human limbal explant. With this method it is possible to obtain up to three limbal epithelial primary cultures from the same explant, thus increasing the efficiency of the in vitro cell culture.

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Change history

  • 22 December 2020

    In the contributors section and chapter 4, the below mentioned affiliation has been added to the authors Ana de la Mata and Marina López Paniagua.

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Acknowledgments

Financial Support: Ministerio de Economía y Competitividad and Fondo Europeo de Desarrollo Regional, Spain (SAF2015-63594-R MINECO/FEDER, EU). Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). Instituto de Salud Carlos III, Spain. Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Spain. 

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Authors and Affiliations

  1. Grupo de Superficie Ocular, Instituto Universitario de Oftalmobiología Aplicada (IOBA), Universidad de Valladolid, Valladolid, Spain

    Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Laura García-Posadas, Ana de la Mata, Rosa M. Corrales, Margarita Calonge & Yolanda Diebold

  2. Centro de Investigación Biomédica en Red de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN). Instituto de Salud Carlos III, Madrid, Spain

    Marina López-Paniagua, Teresa Nieto-Miguel, Sara Galindo, Ana de la Mata, Rosa M. Corrales, Margarita Calonge & Yolanda Diebold

  3. Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, , Valladolid, Spain

    Marina López-Paniagua & Ana de la Mata

Authors
  1. Marina López-Paniagua
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  2. Teresa Nieto-Miguel
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  3. Sara Galindo
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  4. Laura García-Posadas
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  5. Ana de la Mata
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  6. Rosa M. Corrales
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  7. Margarita Calonge
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  8. Yolanda Diebold
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Corresponding author

Correspondence to Yolanda Diebold .

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Editors and Affiliations

  1. Trinity Centre for Biomedical Engineering, Trinity College Dublin, Dublin, Ireland

    Mark Ahearne

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López-Paniagua, M. et al. (2020). Optimization of Human Limbal Stem Cell Culture by Replating a Single Limbal Explant. In: Ahearne, M. (eds) Corneal Regeneration. Methods in Molecular Biology, vol 2145. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0599-8_4

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  • DOI: https://doi.org/10.1007/978-1-0716-0599-8_4

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0598-1

  • Online ISBN: 978-1-0716-0599-8

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