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Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations

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Clinical and Preclinical Models for Maximizing Healthspan

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2138))

Abstract

Disease-associated impairment/dysfunction of stem cell populations is prominent in chronic metabolic and inflammatory diseases, such as type 2 diabetes mellitus (DM) where the multifunctional properties (viability, proliferation, paracrine secretion, multilineage differentiation) of bone marrow resident mesenchymal stem cells (MSCs) can be affected. The growth and viability impairments make it difficult to study the underlying molecular mechanisms related to the dysfunction of these cells in vitro. We have consequently optimized the isolation and culture conditions for impaired/dysfunctional bone marrow MSCs from B6.Cg-Lepob/J obese prediabetic mice. The method described here permits ex vivo investigations into disease-associated functional impairments and the dysregulated molecular mechanisms in these primary MSCs through direct comparisons with their healthy wild-type C57BL6/J control mouse counterparts.

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Acknowledgements

This research was supported by the National Research Foundation (NRF) of South Africa.

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Authors and Affiliations

  1. Department of Medicine, Faculty of Medicine & Health Sciences, Stellenbosch University, Cape Town, South Africa

    Yashar Mehrbani Azar, Maria Jacoba Kruger, Dalene de Swardt, Michelle Maartens, Ascentia Mathapelo Seboko, William Frank Ferris & Mari van de Vyver

Authors
  1. Yashar Mehrbani Azar
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  2. Maria Jacoba Kruger
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  3. Dalene de Swardt
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  4. Michelle Maartens
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  5. Ascentia Mathapelo Seboko
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  6. William Frank Ferris
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  7. Mari van de Vyver
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Corresponding author

Correspondence to Mari van de Vyver .

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Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Mehrbani Azar, Y. et al. (2020). Model for Studying the Effects of Chronic Metabolic Disease on Endogenous Bone Marrow Stem Cell Populations. In: Guest, P. (eds) Clinical and Preclinical Models for Maximizing Healthspan. Methods in Molecular Biology, vol 2138. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0471-7_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0471-7_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0470-0

  • Online ISBN: 978-1-0716-0471-7

  • eBook Packages: Springer Protocols

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