Abstract
A quantum dot (QD)-based lab-on-bead system is a unique tool for multiple analysis of cancer markers in human serum samples by using a flow cytometer. In terms of specificity and sensitivity, this method is comparable with ELISA, the “gold standard” of serological in-clinic detection of single analytes. Fluorescent microspheres encoded with QDs have been used for the quantitative detection of free and total prostate-specific antigen in human serum samples. Developed multiplex assay demonstrates a clear discrimination between serum samples from control subjects and cancer patients. The proposed QD-based method is adaptable and makes it possible to develop numerous clinical tests with decreased duration and cost for early diagnosis of various diseases.
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This study was supported by the Ministry of Education and Science of the Russian Federation, State Contract no. 16.1034.2017/ΠЧ.
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Tsoy, T., Karaulov, A., Nabiev, I., Sukhanova, A. (2020). Multiplexed Detection of Cancer Serum Antigens with a Quantum Dot-Based Lab-on-Bead System. In: Fontes, A., Santos, B. (eds) Quantum Dots. Methods in Molecular Biology, vol 2135. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0463-2_13
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DOI: https://doi.org/10.1007/978-1-0716-0463-2_13
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