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Preparation of Recombinant Siglecs and Identification of Their Ligands

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Lectin Purification and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2132))

Abstract

Siglecs are transmembrane receptor-like vertebrate lectins that recognize glycans containing sialic acid. Most Siglecs also interact with intracellular signal transduction molecules, and modulate immune responses. Recombinant soluble Siglecs fused with the fragment crystallizable (Fc) region of immunoglobulin G (Siglec-Fc) are a versatile tool for the investigation of Siglec functions. We describe protocols for the production of recombinant Siglec-Fc, the analysis of expression of Siglec ligands by flow cytometry, and the identification of the Siglec ligand candidates based on proximity labeling.

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Acknowledgments

This work was supported by the grants from the Ministry of Science and Technology, Taiwan (105-2627-M-007-001 and 104-2311-B-001-017-MY3), Summit Project by Academia Sinica and Ministry of Science and Technology (107-0210-01-19-01), and Summit Project by Academia Sinica (AS-SUMMIT-108), Taiwan.

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Authors and Affiliations

  1. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Lan-Yi Chang, Penk Yeir Low, Deepa Sridharan, Kaia Gerlovin & Takashi Angata

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  1. Lan-Yi Chang
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  2. Penk Yeir Low
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  3. Deepa Sridharan
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  4. Kaia Gerlovin
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  5. Takashi Angata
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Corresponding author

Correspondence to Takashi Angata .

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  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Jun Hirabayashi

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Chang, LY., Low, P.Y., Sridharan, D., Gerlovin, K., Angata, T. (2020). Preparation of Recombinant Siglecs and Identification of Their Ligands. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0430-4_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0429-8

  • Online ISBN: 978-1-0716-0430-4

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