Abstract
Histo-blood group antigen (HBGA) recognition by norovirus (NoV) has been studied using various techniques. Enzyme-linked immunosorbent assays (ELISAs) using virus-like particles (VLPs) have enabled us to visualize the last step of HBGAs-NoV binding with a total reaction time of approximately 8 h. Herein, we describe two ELISA-based methods to detect and quantify NoV VLP attachment to HBGAs: saliva-VLP binding assay and carbohydrate-VLP binding assay.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jiang X, Wang M, Wang K et al (1993) Sequence and genomic organization of Norwalk virus. Virology 195:51–61
Glass PJ, White LJ, Ball JM et al (2000) Norwalk virus open reading frame 3 encodes a minor structural protein. J Virol 74:6581–6591
Prasad BV, Hardy ME, Dokland T et al (1999) X-ray crystallographic structure of the Norwalk virus capsid. Science 286:287–290
Xi JN, Graham DY, Wang KN et al (1990) Norwalk virus genome cloning and characterization. Science 250:1580–1583
Green KY, Lew JF, Jiang X et al (1993) Comparison of the reactivities of baculovirus-expressed recombinant Norwalk virus capsid antigen with those of the native Norwalk virus antigen in serologic assays and some epidemiologic observations. J Clin Microbiol 31:2185–2191
Jiang X, Wang M, Graham DY et al (1992) Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol 66:6527–6532
Prasad BV, Rothnagel R, Jiang X et al (1994) Three-dimensional structure of baculovirus-expressed Norwalk virus capsids. J Virol 68:5117–5125
Harrington PR, Lindesmith L, Yount B et al (2002) Binding of Norwalk virus-like particles to ABH histo-blood group antigens is blocked by antisera from infected human volunteers or experimentally vaccinated mice. J Virol 76:12335–12343
Huang P, Farkas T, Zhong W et al (2005) Norovirus and histo-blood group antigens: demonstration of a wide spectrum of strain specificities and classification of two major binding groups among multiple binding patterns. J Virol 79:6714–6722
Shirato H, Ogawa S, Ito H et al (2008) Noroviruses distinguish between type 1 and type 2 histo-blood group antigens for binding. J Virol 82:10756–10767
Shirato-Horikoshi H, Ogawa S, Wakita T et al (2007) Binding activity of norovirus and sapovirus to histo-blood group antigens. Arch Virol 152:457–461
Choi JM, Hutson AM, Estes MK et al (2008) Atomic resolution structural characterization of recognition of histo-blood group antigens by Norwalk virus. Proc Natl Acad Sci U S A 105:9175–9180
Kubota T, Kumagai A, Ito H et al (2012) Structural basis for the recognition of Lewis antigens by genogroup I norovirus. J Virol 86:11138–11150
(1981) Appendix 2: saliva testing for ABH and Lewis. In: Sidmann FK (ed) Technical manual of the American Association of Blood Banks, 8th edn. Lippincott JB, Philadelphia, PA, pp 122–123
Chakravarty S, Hutson AM, Estes MK et al (2005) Evolutionary trace residues in noroviruses: importance in receptor binding, antigenicity, virion assembly, and strain diversity. J Virol 79:554–568
Tan M, Huang P, Meller J et al (2003) Mutations within the P2 domain of norovirus capsid affect binding to human histo-blood group antigens: evidence for a binding pocket. J Virol 77:12562–12571
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Shirato, H. (2020). ELISA-Based Methods to Detect and Quantify Norovirus Virus-Like Particle Attachment to Histo-Blood Group Antigens. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_51
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0430-4_51
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0429-8
Online ISBN: 978-1-0716-0430-4
eBook Packages: Springer Protocols