Abstract
LecB/PA-IIL (Pfam PF07472) from bacterium Pseudomonas aeruginosa is a fucose-binding lectin with unusual high affinity for glycans. The occurrence of LecB and related proteins is limited to few opportunistic bacterial species, some of them being responsible for severe infections in immune-compromised patients. This lectin is therefore of interest as a target for the design of anti-infectious compounds, but can also be used for research and biotechnology. LecB is a small protein that can be produced in good quantity in recombinant system and purified by affinity chromatography.
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Acknowledgments
The authors acknowledge support by the ANR PIA Glyco@Alps (ANR-15-IDEX-02), Labex ARCANE and CBH-EUR-GS (ANR-17-EURE-0003), and the French Cystic Fibrosis Association Vaincre La Mucoviscidose.
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Gillon, E., Varrot, A., Imberty, A. (2020). LecB, a High Affinity Soluble Fucose-Binding Lectin from Pseudomonas aeruginosa. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_46
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_46
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