Abstract
Pleurotus cornucopiae mycelial lectin (PCL-M) is a divalent cation-dependent GalNAc-specific lectin that is purified from the mycelia of the edible mushroom Pleurotus cornucopiae. PCL-M is produced only in dikaryotic mycelia growing on solid medium and is not present in the mycelia of fruiting bodies. It is a multimeric glycoprotein composed of 40 kDa subunits linked by disulfide bonds. PCL-M is localized on the surface of aggregating mycelia and may be involved in primordia formation. The procedure for PCL-M purification has been discussed herein. Additionally, an experimental method for analyzing the localization of PCL-M on mycelial surface has been described.
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Oguri, S. (2020). Pleurotus cornucopiae Mycelial Lectin (PCL-M): Purification and Detection of the Activity on Mycelial Surface. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_43
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_43
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0429-8
Online ISBN: 978-1-0716-0430-4
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