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Purification of GNA-Related Lectins from Natural Sources

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Lectin Purification and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2132))

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Abstract

The Galanthus nivalis lectin, abbreviated as GNA, is the model protein for a large group of mannose-binding lectins. Here, we describe the purification of GNA starting from dry bulbs. Using a combination of ion exchange chromatography and affinity chromatography on mannose-Sepharose, a highly pure preparation of GNA can be obtained.

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Abbreviations

GNA:

Galanthus nivalis agglutinin

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Acknowledgments

This work was supported mainly by grants from Ghent University and the Fund for Scientific Research-Flanders.

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Authors and Affiliations

  1. Laboratory of Glycobiology and Biochemistry, Department of Biotechnology, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium

    Els J. M. Van Damme

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  1. Els J. M. Van Damme
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Correspondence to Els J. M. Van Damme .

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Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Jun Hirabayashi

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Van Damme, E.J.M. (2020). Purification of GNA-Related Lectins from Natural Sources. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_40

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  • DOI: https://doi.org/10.1007/978-1-0716-0430-4_40

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0429-8

  • Online ISBN: 978-1-0716-0430-4

  • eBook Packages: Springer Protocols

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