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Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host

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Lectin Purification and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2132))

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Abstract

Datura stramonium seeds contain at least three chitin-binding isolectins as homo- or heterodimers of A and B subunits. This lectin has been used for the detection and isolation of sugar chains with N-acetyllactosaminyl structures on highly branched N-glycans. In terms of future diagnostic use, the development of a recombinant lectin will be the most effective approach for producing homogeneous lectin preparations. This chapter presents details of the procedure used for lectin purification and also describes a method that can be used for producing active recombinant homodimeric BB-isolectin in Arabidopsis plants.

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Authors and Affiliations

  1. Department of Northern Biosphere Agriculture, Faculty of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan

    Suguru Oguri

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  1. Suguru Oguri
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Correspondence to Suguru Oguri .

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Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Jun Hirabayashi

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Oguri, S. (2020). Methods for Purifying Datura stramonium Agglutinin and Producing Recombinant Agglutinin Protein in a Heterologous Plant Host. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_32

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  • DOI: https://doi.org/10.1007/978-1-0716-0430-4_32

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0429-8

  • Online ISBN: 978-1-0716-0430-4

  • eBook Packages: Springer Protocols

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