Abstract
Malectin is a membrane-anchored endoplasmic reticulum (ER)-resident lectin, which is first identified in Xenopus laevis in 2008 and highly conserved among animals. Malectin plays an important role in the quality control of glycoprotein in the ER through recognizing the Glc2Man9GlcNAc2 (G2M9) oligosaccharide chain on the newly synthesized glycoproteins. In this chapter, we will describe the preparation of recombinant soluble malectin and its tetramer, which might be developed as useful tools for detection of GlcĪ±1-3Glc containing glycans on the cell surface.
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Acknowledgments
This work was supported by National Natural Science Foundation of China. [81602436], Natural Science Foundation of Guangdong Province [2016A030313102], and the Fundamental Research Funds for the Central Universities [21617495].
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Qin, SY., Hu, D., Yamamoto, K. (2020). Preparation of Soluble Malectin and Its Tetramer. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_28
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_28
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Online ISBN: 978-1-0716-0430-4
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