Abstract
In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels from the family Mytilidae (phylum Mollusca). “MytiLec-1,” the lectin described in this chapter, was the first member of this family to be isolated and characterized from the Mediterranean mussel Mytilus galloprovincialis, a commercially and ecologically important species, spread in marine coastal areas worldwide. MytiLec-1 bound to the sugar moiety of globotriose (Gb3: Galα1-4Galβ1-4Glc), an α-galactoside, leading to apoptosis of Gb3-expressing Burkitt’s lymphoma cells. Although the primary structure of MytiLec-1 was quite unusual, its three-dimensional structure was arranged as a β-trefoil fold, which is the typical architecture of “Ricin B chain (or R)-type” lectins, which are found in a broad range of organisms. To date, MytiLec-1-like lectins have been exclusively found in a few species of the mollusk family Mytilidae (M. galloprovincialis, M. trossulus, M. californianus, and Crenomytilus grayanus) and in the phylum Brachiopoda. Transcriptome data revealed the presence of different structural forms of mytilectin in mussels, which included prototype and chimera-type proteins. The primary sequence of these lectins did not match any previously described known protein family, leading to their assignment to the new “mytilectin family.” We here report the method of purification of this lectin and describe its use in cell biology.
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Acknowledgments
The authors would like to thank Prof. Dr. Yukiko Ogawa (Nagasaki International University, Sasebo, Japan) and Dr. Sultana Rajia (Varendra University, Rajshahi, Bangladesh) for contributing with the cell biological studies described in this chapter. This work was supported in part by a Grant-in-Aid for Scientific Research (KAKENHI JP19K06239 and JP18K0758) from Japan Society for the Promotion of Science (JSPS), a research funding from Sugiyama Chemical and Industrial Laboratory, a grant for academic research from Yokohama City University and Nagasaki International University, a research and developmental support for life sciences from the City of Yokohama (life innovation platform, LIP.Yokohama).
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Fujii, Y., Kawsar, S.M.A., Hasan, I., Fujita, H., Gerdol, M., Ozeki, Y. (2020). Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_21
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DOI: https://doi.org/10.1007/978-1-0716-0430-4_21
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