Abstract
During embryogenesis, beta-cells arise from the dorsal and ventral bud originating in the endoderm germ layer. As the animal develops to adulthood, the beta-cell mass dramatically increases. The expansion of the beta-cell population is driven by cell division among the embryonic beta-cells and supplanted by neogenesis from post-embryonic progenitors. Here, we describe a protocol for multicolor clonal analysis in zebrafish to define the contribution of individual embryonic beta-cells to the increase in cell numbers. This technique provides insights into the proliferative history of individual beta-cells in an islet. This insight helps in defining the replicative heterogeneity among individual beta-cells during development. Additionally, the ability to discriminate individual cells based on unique color signatures helps quantify the volume occupied by beta-cells and define the contribution of cellular size to the beta-cell mass.
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Acknowledgment
We apologize to our colleagues in the field for omitted citations due to restrictions on space and number of references. This work was supported by funding from the DFG–Center for Regenerative Therapies Dresden, Cluster of Excellence at TU Dresden, and the German Center for Diabetes Research (DZD), as well as research grants from the German Research Foundation (DFG), the European Foundation for the Study of Diabetes (EFSD), and the DZD to N.N.
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Singh, S.P., Ninov, N. (2020). Multicolor Labeling and Tracing of Pancreatic Beta-Cell Proliferation in Zebrafish. In: King, A. (eds) Animal Models of Diabetes. Methods in Molecular Biology, vol 2128. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0385-7_12
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DOI: https://doi.org/10.1007/978-1-0716-0385-7_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0384-0
Online ISBN: 978-1-0716-0385-7
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