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Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs

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Expression, Purification, and Structural Biology of Membrane Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2127))

Abstract

Single-particle cryo-electron microscopy has become an indispensable technique in structural biology. In particular when studying membrane proteins, it allows the use of membrane-mimicking tools, which can be crucial for a comprehensive understanding of the structure-function relationship of the protein in its native environment. In this chapter we focus on the application of nanodiscs and use our recent studies on the TMEM16 family as an example.

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Acknowledgments

We thank Hendrik Siekkema and Dawid Deneka for valuable discussion and Jan Rheinberger and Chancievan Thangaratnarajah for critical reading of the manuscript. V.K was funded by the SNF early postdocmobility fellowship 187679. C.P. was funded by the NWO Veni grant 722.017.001 and the NWO Start-Up grant 740.018.016.

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  1. Valeria Kalienkova and Carolina Alvadia contributed equally to this work.

Authors and Affiliations

  1. Department of Structural Biology at the Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands

    Valeria Kalienkova, Vanessa Clerico Mosina & Cristina Paulino

  2. Department of Biochemistry, University of Zurich, Zurich, Switzerland

    Valeria Kalienkova & Carolina Alvadia

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  1. Valeria Kalienkova
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  2. Carolina Alvadia
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Correspondence to Cristina Paulino .

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  1. Biozentrum, University of Basel, Basel, Basel Stadt, Switzerland

    Camilo Perez

  2. Biozentrum, University of Basel, Basel, Basel Stadt, Switzerland

    Timm Maier

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Kalienkova, V., Alvadia, C., Clerico Mosina, V., Paulino, C. (2020). Single-Particle Cryo-EM of Membrane Proteins in Lipid Nanodiscs. In: Perez, C., Maier, T. (eds) Expression, Purification, and Structural Biology of Membrane Proteins. Methods in Molecular Biology, vol 2127. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0373-4_17

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