Skip to main content

Detection of Influenza A Antibodies in Avian Samples by ELISA

  • Protocol
  • First Online:
Animal Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2123))

Abstract

ELISA assays are a fast and relatively inexpensive way to screen sera for antibodies to avian influenza virus. Commercial ELISA kits are available, and although they are more expensive than in-house tests, they provide a ready-to-use assay with good quality control. Numerous sample types can be processed for ELISA: serum, plasma, egg yolk, or blood collected on filter paper. High-quality samples are critical to accurate results. The basics of AIV antibody ELISA, sample processing, result interpretation, and troubleshooting are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Walker DL, Horsfall FL Jr (1950) Lack of identity in neutralizing and hemagglutination-inhibiting antibodies against influenza viruses. J Exp Med 91:65–86

    Article  CAS  Google Scholar 

  2. Spackman E, Pantin-Jackwood M, Swayne D, Suarez DL (2009) An evalution of avian influenza diagnostics methods with domestic duck specimens. Avian Dis 53:276–280

    Article  Google Scholar 

  3. Luo Q, Huang H, Zou W, Dan H, Guo X, Zhang A, Yu Z, Chen H, Jin M (2009) An indirect sandwich ELISA for the detection of avian influenza H5 subtype viruses using anti-hemagglutinin protein monoclonal antibody. Vet Microbiol 137:24–30

    Article  CAS  Google Scholar 

  4. Velumani S, Du Q, Fenner BJ, Prabakaran M, Wee LC, Nuo LY, Kwang J (2008) Development of an antigen-capture ELISA for detection of H7 subtype avian influenza from experimentally infected chickens. J Virol Methods 147:219–225

    Article  CAS  Google Scholar 

  5. Barr IG, McCaig M, Durrant C, Shaw R (2006) The rapid identification of human influenza neuraminidase N1 and N2 subtypes by ELISA. Vaccine 24:6675–6678

    Article  CAS  Google Scholar 

  6. He F, Soejoedono RD, Murtini S, Goutama M, Kwang J (2010) Complementary monoclonal antibody-based dot ELISA for universal detection of H5 avian influenza virus. BMC Microbiol 10:330

    Article  CAS  Google Scholar 

  7. He F, Prabakaran M, Tan Y, Indira K, Kumar SR, Kwang J (2013) Development of dual-function ELISA for effective antigen and antibody detection against H7 avian influenza virus. BMC Microbiol 13:219

    Article  Google Scholar 

  8. Sala G, Cordioli P, Moreno-Martin A, Tollis M, Brocchi E, Piccirillo A, Lavazza A (2003) ELISA test for the detection of influenza H7 antibodies in avian sera. Avian Dis 47:1057–1059

    Article  CAS  Google Scholar 

  9. Moreno A, Brocchi E, Lelli D, Gamba D, Tranquillo M, Cordioli P (2009) Monoclonal antibody based ELISA tests to detect antibodies against neuraminidase subtypes 1, 2 and 3 of avian influenza viruses in avian sera. Vaccine 27:4967–4974

    Article  CAS  Google Scholar 

  10. Kodihalli S, Sivanandan V, Halvorson DA, Nagaraja KV, Kumar MC (1993) Antigen-capture ELISA for rapid diagnosis of avian influenza virus in commercial turkey flocks. J Vet Diagn Investig 5:438–440

    Article  CAS  Google Scholar 

  11. Zhou EM, Chan M, Heckert RA, Riva J, Cantin MF (1998) Evaluation of a competitive ELISA for detection of antibodies against avian influenza virus nucleoprotein. Avian Dis 42:517–522

    Article  CAS  Google Scholar 

  12. Zhang A, Jin M, Liu F, Guo X, Hu Q, Han L, Tan Y, Chen H (2006) Development and evaluation of a DAS-ELISA for rapid detection of avian influenza viruses. Avian Dis 50:325–330

    Article  Google Scholar 

  13. Dusek RJ, Hall JS, Nashold SW, TeSlaa JL, Ip HS (2011) Evaluation of Nobuto filter paper strips for the detection of avian influenza virus antibody in waterfowl. Avian Dis 55:674–676

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Erica Spackman .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2020 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Spackman, E., Killian, M.L. (2020). Detection of Influenza A Antibodies in Avian Samples by ELISA. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 2123. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0346-8_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0346-8_14

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0345-1

  • Online ISBN: 978-1-0716-0346-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics