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Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus

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Animal Influenza Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2123))

Abstract

The agar gel immunodiffusion (AGID) test is used to detect antibodies to Type A influenza group-specific antigens, i.e., the nucleoprotein (NP) and matrix (M) proteins. Therefore, this test will detect antibodies to all influenza A virus subtypes. AGID is commonly used to screen poultry flocks for avian influenza virus infection. The AGID is a simple and economical serological test. All serological testing has its advantages and disadvantages, which should be considered before choosing the optimal test for the laboratory needs. Each laboratory must evaluate the laboratory’s resources, the volume of testing, the goal of testing, how the test results are used, and what types of samples are being tested in order to select the optimal test.

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References

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Correspondence to Terra A. Jenson .

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© 2020 This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

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Jenson, T.A. (2020). Agar Gel Immunodiffusion Assay to Detect Antibodies to Type A Influenza Virus. In: Spackman, E. (eds) Animal Influenza Virus. Methods in Molecular Biology, vol 2123. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0346-8_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0346-8_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0345-1

  • Online ISBN: 978-1-0716-0346-8

  • eBook Packages: Springer Protocols

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