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Protocols for Studying Murine ILC Development

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Innate Lymphoid Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2121))

Abstract

Understanding the origins and developmental trajectory of innate lymphoid cell (ILC) progenitors has been of substantial interest to the fields of ILC biology and immunology. While mature ILC are rare lymphocytes, ILC progenitors represent an even smaller fraction of cells, providing additional challenges in studying them. Moreover, though the approaches to studying these cells are conceptually straightforward, the technical nuances that underlie them can substantially affect the quality of the data. Herein, we provide a detailed protocol for assessing the frequency of ILC progenitors in the bone marrow, their phenotype, and their potential to develop into mature ILC. These methods make up the foundation of in vivo investigations into ILC development, and we hope these thorough protocols and associated notes facilitate additional, high-quality inquiries into this fascinating field.

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Acknowledgments

Optimization of the protocols described herein was supported by the National Institutes of Health (R01AI145265, R01AI124456, R01AI111820, R21AI145397, and U19AI095227) and the US Department of Veterans Affairs (I01BX004299).

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Authors and Affiliations

  1. Division of Allergy, Pulmonary and Critical Care Medicine, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA

    Matthew T. Stier & R. Stokes Peebles Jr

Authors
  1. Matthew T. Stier
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  2. R. Stokes Peebles Jr
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Corresponding author

Correspondence to R. Stokes Peebles Jr .

Editor information

Editors and Affiliations

  1. Laboratory of T cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, UK

    Shoba Amarnath

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Stier, M.T., Peebles, R.S. (2020). Protocols for Studying Murine ILC Development. In: Amarnath, S. (eds) Innate Lymphoid Cells . Methods in Molecular Biology, vol 2121. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0338-3_2

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  • DOI: https://doi.org/10.1007/978-1-0716-0338-3_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0337-6

  • Online ISBN: 978-1-0716-0338-3

  • eBook Packages: Springer Protocols

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