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Group 2 ILC Functional Assays in Allergic Airway Inflammation

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Innate Lymphoid Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2121))

Abstract

ILC2s are a rare innate cell population capable of rapidly producing type 2 cytokines prior to the recruitment and expansion of adaptive type 2 T helper cells. As a result, they are implicated in the pathogenesis of many type-2 immune-mediated diseases, including allergic airway inflammation. Here we describe methods for interrogating and analyzing ILC2 biology in the context of allergic airway inflammation, such as flow cytometric analysis of mouse and human ILC2s as well as live imaging of pulmonary ILC2s.

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Author information

Authors and Affiliations

  1. Inflammation, Repair & Development, National Heart & Lung Institute, Imperial College London, London, UK

    Lewis J. Entwistle, Franz Puttur, Lisa G. Gregory & Clare M. Lloyd

Authors
  1. Lewis J. Entwistle
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  2. Franz Puttur
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  3. Lisa G. Gregory
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  4. Clare M. Lloyd
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Corresponding author

Correspondence to Clare M. Lloyd .

Editor information

Editors and Affiliations

  1. Laboratory of T cell Regulation, Institute of Cellular Medicine, Newcastle University, Newcastle Upon Tyne, UK

    Shoba Amarnath

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Entwistle, L.J., Puttur, F., Gregory, L.G., Lloyd, C.M. (2020). Group 2 ILC Functional Assays in Allergic Airway Inflammation. In: Amarnath, S. (eds) Innate Lymphoid Cells . Methods in Molecular Biology, vol 2121. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0338-3_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0338-3_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0337-6

  • Online ISBN: 978-1-0716-0338-3

  • eBook Packages: Springer Protocols

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