Skip to main content
SpringerLink
Log in

Mechanical Sap Transmission

  • Protocol
  • First Online:
Characterization of Plant Viruses

Part of the book series: Springer Protocols Handbooks ((SPH))

  • 1737 Accesses

Abstract

The transmission of a virus from infected to healthy plant is an important procedure to study the biological properties of the virus. Mechanical transmission is an in vitro method of virus transmission used for a variety of assays such as isolation and propagation of virus isolates, establishing pure culture and sub-culturing of virus isolates, assay for virus infectivity, diagnosis of the virus through indicator hosts, determination of host range and properties of the virus in crude sap and so on. In the laboratory, this is usually accomplished by grinding the leaf of a diseased plant, and rubbing the infectious sap on to the leaf of a healthy plant. The procedure is referred to as mechanical or sap transmission. The different steps and requirements for the process of mechanical transmission are discussed in the chapter.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  • Hill SA (1984) Methods in plant virology. Blackwell Scientific Publications, Oxford

    Google Scholar 

  • Hull R (2002) Matthews’ plant virology, 4th edn. Academic, San Diego

    Chapter  Google Scholar 

  • Hull R (2014) Matthews’ plant virology, 5th edn. Academic, San Diego

    Google Scholar 

  • Kado CI (1972) Mechanical and biological inoculation principles. In: Kado CI, Agrawal HO (eds) Principles and techniques in plant virology. Van Nostrand Reinhold Company, New York

    Google Scholar 

  • Mandal B, Csinos AS, Martinez-Ochoa N, Pappu HR (2008) A rapid and efficient inoculation method for tomato spotted wilt tospovirus. J Virol Methods 149:195–198

    Article  CAS  Google Scholar 

  • Walkey DGA (1991) Applied plant virology. Springer, Dordrecht

    Book  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Crop Protection, ICAR-Indian Institute of Spices Research, Kozhikode, Kerala, India

    Alangar Ishwara Bhat

  2. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India

    Govind Pratap Rao

Authors
  1. Alangar Ishwara Bhat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Govind Pratap Rao
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 2020 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Bhat, A.I., Rao, G.P. (2020). Mechanical Sap Transmission. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-0716-0334-5_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0333-8

  • Online ISBN: 978-1-0716-0334-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation