Abstract
Loop-mediated isothermal amplification (LAMP) can amplify nucleic acid with high specificity, sensitivity and speed under isothermal conditions. The LAMP method uses four to six primers and a DNA polymerase with strand-displacing activity to generate amplification products. The products can be detected by agarose gel electrophoresis or visually by turbidity or colour changes. The amplified products of LAMP are also visible as ladder-like patterns on a gel. LAMP methods have been used successfully for detecting both the RNA and DNA plant viruses.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abdullahi UF, Rochman N, Wan R, Wan T, Ahmadu S, Anas M, Aliyu S, Baig A (2015) Loop-mediated isothermal amplification (LAMP), an innovation in gene amplification: bridging the gap in molecular diagnostics: a review. Indian J Sci and Tech 8:1–12
Bhat AI, Siljo A, Deeshma KP (2013) Rapid detection of Piper yellow mottle virus and Cucumber mosaic virus infecting black pepper (Piper nigrum) by loop-mediated isothermal amplification (LAMP). J Virol Methods 193:190–196
Fukuta S, Ohishi K, Yoshida K, Mizukami Y, Ishida A, Kanbe M (2004) Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of Tomato spotted wilt virus from chrysanthemum. J Virol Methods 121:49–55
Keizerweerda AT, Chandra A, Grisham MP (2015) Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Sugarcane mosaic virus and Sorghum mosaic virus in sugarcane. J Virol Methods 212:23–29
Mori Y, Nagamine K, Tomita N, Notomi T (2001) Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154
Nagamine K, Hase T, Notomi T (2002) Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223–229
Nie X (2005) Reverse transcription loop-mediated isothermal amplification of DNA for detection of Potato virus Y. Plant Dis 89:605–610
Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T (2000) Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63
Siljo A, Bhat AI (2014) Reverse transcription loop-mediated isothermal amplification assay for rapid and sensitive detection of Banana bract mosaic virus in cardamom (Elettaria cardamomum). Eur J Plant Pathol 138:209–214
Tomita N, Mori Y, Kanda H, Notomi T (2008) Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3:877–882
Zhang X, Lowe SB, Gooding JJ (2014) Brief review of monitoring methods for loop-mediated isothermal amplification (LAMP). Biosens Bioelectron 61:491–499
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2020 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bhat, A.I., Rao, G.P. (2020). Loop-Mediated Isothermal Amplification (LAMP). In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_38
Download citation
DOI: https://doi.org/10.1007/978-1-0716-0334-5_38
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0333-8
Online ISBN: 978-1-0716-0334-5
eBook Packages: Springer Protocols