Abstract
All single-stranded RNA (ssRNA) virus-infected plants produce double-stranded RNA (dsRNA) during their replication cycle. dsRNAs are more stable than its counterpart ssRNA. Many protocols are available for the isolation of dsRNA from virus-infected plants that are used as template for cDNA synthesis followed by PCR assay. In this chapter, suitable protocol for the isolation of ds RNA from plant sources is discussed.
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References
Dodds JA, Morris TJ, Jordan RL (1984) Plant viral double stranded RNA. Annu Rev Phytopathol 22:151–168
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Morris TJ, Dodds JA, Hillman B, Jordon R, Lommel SA, Tamaki SJ (1983) Viral specific dsRNA. Diagnostic value for plant virus disease identification. Plant Mol Biol Rep 1:27–30
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Bhat, A.I., Rao, G.P. (2020). Isolation of Double-Stranded (ds) RNA from Virus-Infected Plants. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_33
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DOI: https://doi.org/10.1007/978-1-0716-0334-5_33
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0333-8
Online ISBN: 978-1-0716-0334-5
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