Abstract
Spectrometry is one of the methods used to check the purity of purified virus preparation. In this method, optical density value of purified virus preparation at 260 and 280 nm and absorption spectra from 245 to 300 nm is determined using a spectrometer. The UV spectra and A 260/280 ratio provide information on the purity of the virus preparation. A 260 nm value can be used to estimate the yield of purified virus.
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References
Noordam D (1973) Identification of plant viruses. Methods and experiments. Centre for Agricultural Publishing and Documentation, Wageningen
Wilson K, Goulding KH (1986) Biologists guide to principles and techniques of practical biochemistry. Edward Arnold, London
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Bhat, A.I., Rao, G.P. (2020). Ultraviolet Absorption Spectra of Purified Virus Preparation. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_22
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DOI: https://doi.org/10.1007/978-1-0716-0334-5_22
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0333-8
Online ISBN: 978-1-0716-0334-5
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