Abstract
Glasshouse is an essential requirement for plant virology works. The purpose of the glasshouse is to provide a regulated and controlled environment for maintenance of virus-free and virus-infected plants and propagation of virus in susceptible hosts and to carry out transmission experiments under insect-proof conditions. This chapter provides information on some basic requirements and precautions essential for every plant virus worker to raise and maintain healthy nursery and virus cultures to carry out experiments under regulated environment and to prevent contamination.
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References
Dijkstra J, de Jager CP (1998) Practical plant virology: protocols and exercises. Springer, Amsterdam
Hull R (2002) Matthews’ plant virology, 4th edn. Matthews Academic, San Diego
Maramorosch K, Mahmood F (2014) Rearing animal and plant pathogen vectors. CRC Press, Boca Raton
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Bhat, A.I., Rao, G.P. (2020). Glasshouse for Maintenance of Virus and Insect Culture. In: Characterization of Plant Viruses . Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0334-5_1
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DOI: https://doi.org/10.1007/978-1-0716-0334-5_1
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0333-8
Online ISBN: 978-1-0716-0334-5
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