Abstract
Chemically induced cell fate conversion, including reprogramming to pluripotent stem cells and direct reprogramming to somatic cells, has been proved to be an alternative strategy with many advantages, in comparison with conventional transcription factors- or microRNAs-enabled cell reprogramming. Many functional and desirable cells have been generated via the chemically induced reprogramming. Neural stem cells (NSCs) hold great potential in basic research and clinical application. Here, we describe a detailed protocol for converting mouse fibroblasts into NSCs by a cocktail of chemical compounds.
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Acknowledgments
This work has been supported by the National Natural Science Foundation of China (31871498), the Medical-Engineering Crossover Fund of Shanghai Jiao Tong University (YG2017MS59), Shanghai Collaborative Innovation Program on Regenerative Medicine and Stem Cell Research (2019CXJQ01).
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Wei, C., Xiong, S., Cheng, L. (2020). Reprogramming of Fibroblasts to Neural Stem Cells by a Chemical Cocktail. In: Kidder, B. (eds) Stem Cell Transcriptional Networks. Methods in Molecular Biology, vol 2117. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0301-7_16
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DOI: https://doi.org/10.1007/978-1-0716-0301-7_16
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