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PAMAM Dendrimers as a Delivery System for Small Interfering RNA

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RNA Interference and CRISPR Technologies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2115))

Abstract

Polyamidoamine dendrimers (PAMAM) form positively charged nanoparticles that function as nonviral delivery vectors for gene therapy. They protect nucleic acids from enzymatic degradation and facilitate endocytosis and endosomal escape. In this chapter, we describe the preparation and in vitro evaluation of small interfering RNA (siRNA)-PAMAM dendrimers. The physicochemical properties of the designed formulations were evaluated by size and zeta potential assessment and atomic force microscopy (AFM). The binding and release of the siRNA molecules from the PAMAM dendrimers were also assessed. Visualization and quantitative analysis of the siRNA-PAMAM dendrimers in live cells were analyzed by fluorescence microscopy and flow cytometry, respectively. Improving siRNA delivery to human cells through PAMAM dendrimers should accelerate the clinical applications of RNA interference.

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References

  1. Ayatollahi S, Salmasi Z, Hashemi M, Askarian S, Oskuee RK, Abnous K, Ramezani M (2017) Aptamer-targeted delivery of Bcl-xL shRNA using alkyl modified PAMAM dendrimers into lung cancer cells. Int J Biochem Cell Biol 92:210–217

    Article  CAS  Google Scholar 

  2. Gu J, Fang X, Hao J, Sha X (2015) Reversal of P-glycoprotein-mediated multidrug resistance by CD44 antibody-targeted nanocomplexes for short hairpin RNA-encoding plasmid DNA delivery. Biomaterials 45:99–114

    Article  CAS  Google Scholar 

  3. Chang PK, Prestidge CA, Bremmell KE (2017) Interfacial analysis of siRNA complexes with poly-ethylenimine (PEI) or PAMAM dendrimers in gene delivery. Colloids Surf B: Biointerfaces 158:370–378

    Article  CAS  Google Scholar 

  4. Abedi-Gaballu F, Dehghan G, Ghaffari M, Yekta R, Abbaspour-Ravasjani S, Baradaran B, Dolatabadi JEN, Hamblin MR (2018) PAMAM dendrimers as efficient drug and gene delivery nanosystems for cancer therapy. Appl Mater Today 12:177–190

    Article  Google Scholar 

  5. Ghaffari M, Dehghan G, Abedi-Gaballu F, Kashanian S, Baradaran B, Ezzati Nazhad Dolatabadi J, Losic D (2018) Surface functionalized dendrimers as controlled-release delivery nanosystems for tumor targeting. Eur J Pharm Sci 122:11–330

    Article  Google Scholar 

  6. Ezzati Nazhad Dolatabadi J, Omidi Y (2016) Solid lipid-based nanocarriers as efficient targeted drug and gene delivery systems. TrAC Trends Anal Chem 77:100–108

    Article  CAS  Google Scholar 

  7. Ezzati Nazhad Dolatabadi J, Omidi Y, Losic D (2011) Carbon nanotubes as an advanced drug and gene delivery nanosystem. Curr Nanosci 7:297–314

    Article  Google Scholar 

  8. Pandi P et al (2018) Dendrimer as a new potential carrier for topical delivery of siRNA: comparative study of dendriplex vs. lipoplex for delivery of TNF-α siRNA. Int J Pharm 550(1–2):240–250

    Article  CAS  Google Scholar 

  9. Perez A, Romero E, Morilla M (2009) Ethylendiamine core PAMAM dendrimers/siRNA complexes as in vitro silencing agents. Int J Pharm 380(1–2):189–200

    Article  CAS  Google Scholar 

  10. Wang Y, Fang J, Cheng D, Wang Y, Shuai X (2014) A pH-sensitive micelle for codelivery of siRNA and doxorubicin to hepatoma cells. Polymer 55:3217–3226

    Article  CAS  Google Scholar 

  11. Zhou J, Neff CP, Liu X, Zhang J, Li H, Smith DD, Swiderski P, Aboellail T, Huang Y, Du Q, Liang Z, Peng L, Akkina R, Rossi JJ (2011) Systemic administration of combinatorial dsiRNAs via nanoparticles efficiently suppresses HIV-1 infection in humanized mice. Mol Ther 19:2228–2238

    Article  CAS  Google Scholar 

  12. Zhang Y, Wang Z, Gemeinhart RA (2013) Progress in microRNA delivery. J Control Release 172:962–974

    Article  CAS  Google Scholar 

  13. Shan Y, Luo T, Peng C, Sheng R, Cao A, Cao X, Shen M, Guo R, Tomás H, Shi X (2012) Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors. Biomaterials 33:3025–3035

    Article  CAS  Google Scholar 

  14. Tariq I, Pinnapireddy SR, Duse L, Ali MY, Ali S, Amin MU, Goergen N, Jedelská J, Schäfer J, Bakowsky U (2018) Lipodendriplexes: A promising nanocarrier for enhanced gene delivery with minimal cytotoxicity. Eur J Pharm Biopharm 135:72–82

    Article  Google Scholar 

  15. Ma D, Lin QM, Zhang LM, Liang YY, Xue W (2014) A star-shaped porphyrin-arginine functionalized poly(L-lysine) copolymer for photo-enhanced drug and gene co-delivery. Biomaterials 35:4357–4367

    Article  CAS  Google Scholar 

  16. Zarebkohan A, Najafi F, Moghimi HR, Hemmati M, Deevband MR, Kazemi B (2015) Synthesis and characterization of a PAMAM dendrimer nanocarrier functionalized by SRL peptide for targeted gene delivery to the brain. Eur J Pharm Sci 12:19–30

    Article  Google Scholar 

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Authors and Affiliations

  1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Shiva Kheiriabad & Maryam Ghaffari

  2. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    Jafar Ezzati Nazhad Dolatabadi

  3. Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA, USA

    Michael R. Hamblin

  4. Department of Dermatology, Harvard Medical School, Boston, MA, USA

    Michael R. Hamblin

  5. Laser Research Centre, Faculty of Health Science, University of Johannesburg, Doornfontein, South Africa

    Michael R. Hamblin

Authors
  1. Shiva Kheiriabad
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  2. Maryam Ghaffari
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  3. Jafar Ezzati Nazhad Dolatabadi
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  4. Michael R. Hamblin
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Corresponding author

Correspondence to Michael R. Hamblin .

Editor information

Editors and Affiliations

  1. Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital-Radiumhospitalet, Oslo, Norway

    Mouldy Sioud

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Kheiriabad, S., Ghaffari, M., Dolatabadi, J.E.N., Hamblin, M.R. (2020). PAMAM Dendrimers as a Delivery System for Small Interfering RNA. In: Sioud, M. (eds) RNA Interference and CRISPR Technologies. Methods in Molecular Biology, vol 2115. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0290-4_5

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  • DOI: https://doi.org/10.1007/978-1-0716-0290-4_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0289-8

  • Online ISBN: 978-1-0716-0290-4

  • eBook Packages: Springer Protocols

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