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Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes

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RNA Spectroscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2113))

Abstract

Native electrospray ionization mass spectrometry (native ESI-MS) is a powerful tool to investigate non-covalent biomolecular interactions. It has been widely used to study protein complexes, but only few examples are described for the analysis of complexes involving RNA-RNA interactions. Here, we provide a detailed protocol for native ESI-MS analysis of RNA complexes. As an example, we present the analysis of the HIV-1 genomic RNA dimerization initiation site (DIS) extended duplex dimer bound to the aminoglycoside antibiotic lividomycin.

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Acknowledgments

Authors would like to thank Dominique Burnouf for critical reading of the manuscript and useful comments and are grateful to Philippe Dumas for his support.

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Authors and Affiliations

  1. Architecture et Réactivité de l’ARN - CNRS UPR 9002, Institut de Biologie Moléculaire et Cellulaire, Université de Strasbourg, Strasbourg, France

    Philippe Wolff & Eric Ennifar

  2. Plateforme protéomique Strasbourg Esplanade, FRC1589 du CNRS, Université de Strasbourg, Strasbourg, France

    Philippe Wolff

Authors
  1. Philippe Wolff
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  2. Eric Ennifar
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Corresponding author

Correspondence to Philippe Wolff .

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Editors and Affiliations

  1. Université de Paris, Paris, France, Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, Gif-sur-Yvette, France

    Véronique Arluison

  2. Synchrotron SOLEIL L’Orme des Merisiers Saint Aubin, Gif-sur-Yvette, France

    Frank Wien

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Wolff, P., Ennifar, E. (2020). Native Electrospray Ionization Mass Spectrometry of RNA-Ligand Complexes. In: Arluison, V., Wien, F. (eds) RNA Spectroscopy. Methods in Molecular Biology, vol 2113. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0278-2_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0278-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0277-5

  • Online ISBN: 978-1-0716-0278-2

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