Abstract
Colocalization single-molecule spectroscopy (CoSMoS) allows studying RNA-protein complexes in the full complexity of their cellular environment at single-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe an approach to monitor multiple RNA species simultaneously to enable direct comparison. This approach represents a technological development to avoid conventional inter-experiment comparisons.
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Acknowledgments
Joerg E. Braun acknowledges funding from the Human Frontier Science Program (HFSP) LT000166/2013 and the European Molecular Biology Organization (EMBO) ALTF 890-2012. Joerg E. Braun is supported by funding to the laboratory of Melissa J. Moore: NIH R01 GM053007.
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Haraszti, R.A., Braun, J.E. (2020). Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species. In: Arluison, V., Wien, F. (eds) RNA Spectroscopy. Methods in Molecular Biology, vol 2113. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0278-2_3
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DOI: https://doi.org/10.1007/978-1-0716-0278-2_3
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