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Application of NIR Raman Spectroscopy to Probe the Flexibility of RNA Structure

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RNA Spectroscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2113))

Abstract

NIR Raman spectroscopy has great potential for the detection of very weak Stokes-shifted Raman scattering emitted by biomolecules. Reports relating Raman spectroscopy analyses of ribonucleic acids (RNAs) are minimal. Nevertheless, correlation between Raman vibrational spectra and specific structural features of RNA from Avocado sunblotch viroids (ASBVds) has been clearly established. In this chapter, we discuss how to obtain NIR Raman RNA spectra and applications of Raman spectroscopy for the structural analysis of RNA, based on the analysis of ASBVd RNA. This chapter includes the analysis of spectral changes upon thermal denaturation, deuteration, and self-cleavage perturbations.

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Acknowledgments

Marie-Christine Maurel from UPMC Université, Paris 6, France, is thanked for initiating and planning this work. Jacques Verne and Hussein Kaddour are thanked for plasmid constructions and purification of the ASBVds. Hussein Kaddour who was the recipient of a fellowship from the Center National de la Recherche Scientifique (CNRS) did most of the Raman experiments. Sergei Kruglik from UPMC, Paris 6 and Sorbonne Universités, Laboratoire Jean-Perrin, 75005 Paris, is thanked for initiating the NIR Raman spectroscopy and all the data processing. This study was supported by Université Paris 6, the CNRS (grant PID EPOV), and the Centre National d’Etudes Spatiale. Richard Sinden is thanked for careful reading and helpful comments on this manuscript.

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Authors and Affiliations

  1. INSERM, Fontenay Aux Roses, France

    Hui Bon Hoa Gaston

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  1. Hui Bon Hoa Gaston
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Editors and Affiliations

  1. Université de Paris, Paris, France, Laboratoire Léon Brillouin LLB, CEA, CNRS UMR12, Université Paris Saclay, Gif-sur-Yvette, France

    Véronique Arluison

  2. Synchrotron SOLEIL L’Orme des Merisiers Saint Aubin, Gif-sur-Yvette, France

    Frank Wien

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Gaston, H.B.H. (2020). Application of NIR Raman Spectroscopy to Probe the Flexibility of RNA Structure. In: Arluison, V., Wien, F. (eds) RNA Spectroscopy. Methods in Molecular Biology, vol 2113. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0278-2_12

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  • DOI: https://doi.org/10.1007/978-1-0716-0278-2_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0277-5

  • Online ISBN: 978-1-0716-0278-2

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