Abstract
Single-cell transcriptomic analysis has become a new and powerful tool to study complex multicellular systems. Single-cell RNA sequencing provides an unbiased classification of heterogeneous cellular states at the transcriptional level, but it does not always correlate to cell-surface protein expression. A recently developed method called cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq) simultaneously measures surface proteins and gene expression from single cells. Briefly, based on the existing single-cell sequencing technology, oligonucleotide-labeled antibodies and barcoded primer gel beads are used to bind to corresponding cell-surface proteins and mRNA, respectively. Further, libraries of labeled protein and RNA information are sequenced to integrate cellular protein and transcriptome reads together efficiently. CITE-seq is transforming comprehensive genomic studies into models of causal gene-protein investigation.
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Acknowledgement
This work was supported by NIH grant R01HL137709.
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Luo, J., Erb, C.A., Chen, K. (2020). Simultaneous Measurement of Surface Proteins and Gene Expression from Single Cells. In: Liu, C. (eds) T-Cell Receptor Signaling. Methods in Molecular Biology, vol 2111. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0266-9_3
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DOI: https://doi.org/10.1007/978-1-0716-0266-9_3
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Publisher Name: Humana, New York, NY
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