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An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides

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Peptide Synthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2103))

Abstract

Microwave technology, in conjunction with the isopeptide strategy including Fmoc-based solid-phase peptide synthesis (SPPS), was used to establish a methodology for time-efficient synthesis of peptides containing difficult sequences. A model difficult sequence-containing peptide (8QSer) was synthesized through this method in 1 day, representing a tenfold reduction in synthesis time compared to the isopeptide method combined with classical SPPS.

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Author information

Authors and Affiliations

  1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia

    Waleed M. Hussein, Mariusz Skwarczynski & Istvan Toth

  2. Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD, Australia

    Istvan Toth

  3. School of Pharmacy, The University of Queensland, Woolloongabba, QLD, Australia

    Istvan Toth

Authors
  1. Waleed M. Hussein
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  2. Mariusz Skwarczynski
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  3. Istvan Toth
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Corresponding author

Correspondence to Istvan Toth .

Editor information

Editors and Affiliations

  1. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia

    Waleed M. Hussein

  2. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia

    Mariusz Skwarczynski

  3. School of Chemistry and Molecular Biosciences, The University of Queensland, St. Lucia, QLD, Australia

    Istvan Toth

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Hussein, W.M., Skwarczynski, M., Toth, I. (2020). An Isodipeptide Building Block for Microwave-Assisted Solid-Phase Synthesis of Difficult Sequence-Containing Peptides. In: Hussein, W., Skwarczynski, M., Toth, I. (eds) Peptide Synthesis. Methods in Molecular Biology, vol 2103. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0227-0_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0227-0_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0226-3

  • Online ISBN: 978-1-0716-0227-0

  • eBook Packages: Springer Protocols

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