Abstract
Dishomeostasis of Cu(II) ions in the human body is connected with several serious diseases such as Alzheimer’s disease or Wilson’s disease. Therefore, a deep understanding of Cu(II)-binding properties to metal ions carriers, together with the knowledge about how they can interact with other copper-binding partners, e.g., amyloid-β (Aβ), is required to assess their relevance to the brain metal homeostasis. Ultraviolet-visible spectrometry (UV-Vis) and circular dichroism (CD) were used to study the coordination characteristics of Cu(II) with peptide containing the amino-terminal (H2N–Xaa–Yaa–His–) copper-binding (ATCUN) motif (Aβ12–16—VHHQK-NH2) derived from Aβ peptide.
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Acknowledgments
The National Science Centre Poland (ETIUDA grant, UMO-2016/20/T/ST4/00334, and PRELUDIUM grant, UMO-2014/13/N/ST5/01553) and University of Melbourne are gratefully acknowledged for financial support in the form of scholarships to Mariusz Mital. The authors are also thankful to Peishan Chen.
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Mital, M., Sęk, J.P., Ziora, Z.M. (2020). Metal–Peptide Complexes to Study Neurodegenerative Diseases. In: Hussein, W., Skwarczynski, M., Toth, I. (eds) Peptide Synthesis. Methods in Molecular Biology, vol 2103. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0227-0_22
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DOI: https://doi.org/10.1007/978-1-0716-0227-0_22
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