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Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media

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Immobilization of Enzymes and Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2100))

Abstract

Adsorption of lipases on hydrophobic supports is a very easy immobilization protocol and it yields very interesting immobilized lipase derivatives. The open and active form of lipase molecules becomes stabilized by strong adsorption on the support surface. By using very rigid hydrophobic supports (e.g., methacrylate), lipase derivatives are very useful to catalyze biotransformations in fully anhydrous organic media (solvents, solvent-free systems, etc.) and design of continuous flow reactors. In addition to that, the design of different lipase derivatives allows the modulation of functional properties of the derivatives. In this chapter, methodology of immobilization into hydrophobic carriers is described using as case study the preparation of immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL), and the following particular features will be discussed:

  1. 1.

    Adsorption on hydrophobic supports yields lipase derivatives that are much more active and stable than other immobilized lipase derivatives.

  2. 2.

    Regioselectivity can be modulated, for example, TLL adsorbed on divinyl benzene hydrophobic supports retains a 1,3 regioselectivity during ethanolysis of oils. On the contrary, the enzyme adsorbed on octadecyl supports loses the regioselectivity and allows the complete ethanolysis of oils (e.g., biodiesel synthesis).

  3. 3.

    TLL adsorbed on octadecyl supports with large pore size (60 nm) is tenfold more active for ethanolysis in solvent-free systems than TLL derivatives adsorbed on supports with small pore size (10 nm).

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Author information

Authors and Affiliations

  1. Department of Biotechnology and Microbiology, Institute of Food Science Research (CIAL), CSIC-UAM, Campus UAM, Madrid, Spain

    Gloria Fernandez-Lorente

  2. Department of Biocatalysis, Institute of Catalysis and Petrochemistry (ICP) CSIC, Campus UAM, Madrid, Spain

    Gloria Fernandez-Lorente, Javier Rocha-Martín & Jose M. Guisan

Authors
  1. Gloria Fernandez-Lorente
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  2. Javier Rocha-Martín
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  3. Jose M. Guisan
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Corresponding author

Correspondence to Jose M. Guisan .

Editor information

Editors and Affiliations

  1. CSIC Campus Cantoblanco, Instituto de Catalisis, Madrid, Spain

    Jose M. Guisan

  2. Institute of Biotech and Biochemical Engineering, Graz University of Technology, Graz, Austria

    Juan M. Bolivar

  3. CIC biomaGUNE, Donostia-San Sebastian, Spain

    Fernando López-Gallego

  4. Campus UAM-CSIC, Institute of Catatalysis, Madrid, Spain

    Javier Rocha-Martín

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Fernandez-Lorente, G., Rocha-Martín, J., Guisan, J.M. (2020). Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media. In: Guisan, J., Bolivar, J., López-Gallego, F., Rocha-Martín, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0215-7_9

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  • DOI: https://doi.org/10.1007/978-1-0716-0215-7_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0214-0

  • Online ISBN: 978-1-0716-0215-7

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