Abstract
In this chapter, the properties of tailor-made anionic exchanger resins based on films of large polyethylenimine polymers (e.g., molecular weight 25,000) as supports for strong but reversible immobilization of proteins are shown. The polymer is completely coated, via covalent immobilization, the surface of different porous supports. Proteins can interact with this polymeric bed, involving a large percentage of the protein surface in the adsorption. Different enzymes have been very strongly adsorbed on these supports, retaining enzyme activities. On the other hand, adsorption is very strong and the derivatives may be used under a wide range of pH and ionic strengths. These supports may be useful even to stabilize multimeric enzymes, by involving several enzyme subunits in the immobilization.
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References
Rosevear A (1984) Immobilized biocatalysts: a critical review. J Chem Technol Biotechnol 34:127–150
Royer GP (1980) Immobilized enzymes as catalysts. Catal Rev 22:29–73
Klivanov AM (1983) Immobilized enzymes and cells as practical catalysts. Science 219:722–727
Hartmeier W (1985) Immobilized biocatalysts: from simple to complex systems. Trends Biotechnol 3:149–153
Kennedy JF, Melo EHM, Jumel K (1990) Immobilized enzymes and cells. Chem Eng Prog 45:81–89
Katchalski-Katzir E (1993) Immobilized enzymes: learning from past successes and failures. Trends Biotechnol 11:471–478
Chibata I, Tosa T, Sato T (1986) Biocatalysis: immobilized cells and enzymes. J Mol Catal 37:1–24
Gupta MN (1991) Thermostabilization of proteins. Biotechnol Appl Biochem 14:1–11
Mateo C, Abian O, Fernández-Lafuente R, Guisán JM (2000) Reversible enzyme immobilization via a very strong and nondistorting ionic adsorption on support Polyethylenimine supports. Biotechnol Bioeng 7:98–105
Pessela BCC, Fernández-Lafuente R, Fuentes M, Vián A, García JL, Carrascosa AV, Mateo C, Guisán JM (2003) Reversible immobilization of a thermophilic β-galactosidase via ionic adsorption on PEI- coated sepabeads. Enzym Microb Technol 32:369–374
Fuentes M, Maquiese J, Pessela BCC, Abian A, Fernández-Lafuente R, Mateo C, Guisán JM (2004) New cationic exchanger support for reversible immobilization of proteins. Biotechnol Prog 20:284–288
Fuentes M, Pessela BCC, Maquiese JV, Ortiz C, Segura RL, Palomo JM, Abian O, Torres R, Mateo C, Fernández-Lafuente R, Guisán JM (2004) Reversible and strong immobilization of proteins by ionic exchange on supports coated with sulfate- dextran. Biotechnol Prog 20:1134–1139
Virgen-Ortíz JJ, Dos Santos JCS, Berenguer-Murcia Á, Barbosa O, Rodrigues RC, Fernandez-Lafuente R (2017) Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts. J Mater Chem B 5(36):7461–7490
Garcia-Galan C, Berenguer-Murcia A, Fernandez-Lafuente R, Rodrigues RC (2011) Potential of different enzyme immobilization strategies to improve enzyme performance. Adv Synth Catal 353(16):2885–2904
Jesionowski T, Zdarta J, Krajewska B (2014) Enzyme immobilization by adsorption: a review. Adsorption 20(5-6):801–821
Batista-Viera F, Barbieri M, Ovsejevi K, Manta C, Carlsson J (1991) A new method for reversible immobilization of thiol biomolecules based on solid-phase bound thiosulfonate groups. Appl Biochem Biotechnol 31:175–195
Batista-Viera F, Brena B, Luna B (1988) Reversible immobilization of soybean amylase on phenylboronate-agarose. Biotechnol Bioeng 31:711–713
Brena B, Ovsejevi K, Luna B, Batista-Viera F (1993) Thiolation and reversible immobilization of sweet potato amylase on thiosulfonate agarose. J Mol Catal 84:381–390
Chibata I, Tosa T (1976) Industrial applications of immobilized enzymes and immobilized microbial cells. In: Applied biochemistry and bioengineering: immobilized enzyme principles, vol 1. Wingard, Katchalski, Goldstein, London, pp 239–260
Torres R, Pessela BCC, Mateo C, Ortiz C, Fuentes M, Guisán JM, Fernández-Lafuente R (2004) Reversible immobilization of glucoamylase by ionic adsorption on sepabeads coated with polyethyleneimine. Biotechnol Prog 20:1297–1300
Tammi M, Ballou L, Taylor A, Ballou C (1987) Effect of glycosylation on yeast invertase oligomer stability. J Biol Chem 262:4395–4401
Chu FK, Watorek W, Maley F (1983) Factors affecting the oligomeric structure of yeast external invertase. Arch Biochem Biophys 223:543–555
Reddy AV, MacColl R, Maley F (1990) Effect of oligosaccharides on oligomeric structures of external, internal and deglycosylated invertase. Biochemistry 29:2482–2487
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Mateo, C. et al. (2020). Very Strong but Reversible Immobilization of Enzymes on Supports Coated with Ionic Polymers. In: Guisan, J., Bolivar, J., López-Gallego, F., Rocha-Martín, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 2100. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0215-7_8
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DOI: https://doi.org/10.1007/978-1-0716-0215-7_8
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