Abstract
Mucosal-associated invariant T (MAIT) cells recognize intermediates of the vitamin B2 biosynthetic pathway present in a variety of bacteria, presented by the monomorphic MR1 molecules. Because of their central role in shaping adaptive immunity through interaction with dendritic cells (DCs) and B cells, their manipulation can be of translational relevance. We describe a method to routinely isolate and maintain MAIT cells from peripheral blood and to investigate their activity using DC as targets.
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References
Salio M, Cerundolo V (2015) Regulation of lipid specific and vitamin specific non-MHC restricted T cells by antigen presenting cells and their therapeutic potentials. Front Immunol 6:388. https://doi.org/10.3389/fimmu.2015.00388
Kjer-Nielsen L, Patel O, Corbett AJ, Le Nours J, Meehan B, Liu L, Bhati M, Chen Z, Kostenko L, Reantragoon R, Williamson NA, Purcell AW, Dudek NL, McConville MJ, O’Hair RA, Khairallah GN, Godfrey DI, Fairlie DP, Rossjohn J, McCluskey J (2012) MR1 presents microbial vitamin B metabolites to MAIT cells. Nature 491:717–723. https://doi.org/10.1038/nature11605
McWilliam HE, Eckle SB, Theodossis A, Liu L, Chen Z, Wubben JM, Fairlie DP, Strugnell RA, Mintern JD, McCluskey J, Rossjohn J, Villadangos JA (2016) The intracellular pathway for the presentation of vitamin B-related antigens by the antigen-presenting molecule MR1. Nat Immunol 17:531–537. https://doi.org/10.1038/ni.3416
Hartmann N, Harriff MJ, McMurtrey CP, Hildebrand WH, Lewinsohn DM, Kronenberg M (2018) Role of MAIT cells in pulmonary bacterial infection. Mol Immunol 101:155–159. https://doi.org/10.1016/j.molimm.2018.06.270
Ussher JE, Bilton M, Attwod E, Shadwell J, Richardson R, de Lara C, Mettke E, Kurioka A, Hansen TH, Klenerman P, Willberg CB (2014) CD161++ CD8+ T cells, including the MAIT cell subset, are specifically activated by IL-12+IL-18 in a TCR-independent manner. Eur J Immunol 44:195–203. https://doi.org/10.1002/eji.201343509
Wilgenburg BV, Loh L, Chen Z, Pediongco TJ, Wang H, Shi M, Zhao Z, Koutsakos M, Nussing S, Sant S, Wang Z, D’Souza C, Jia X, Almeida CF, Kostenko L, Eckle SBG, Meehan BS, Kallies A, Godfrey DI, Reading PC, Corbett AJ, McCluskey J, Klenerman P, Kedzierska K, Hinks TSC (2018) MAIT cells contribute to protection against lethal influenza infection in vivo. Nat Commun 9:4706. https://doi.org/10.1038/s41467-018-07207-9
Salio M, Gasser O, Gonzalez-Lopez C, Martens A, Veerapen N, Gileadi U, Verter JG, Napolitani G, Anderson R, Painter G, Besra GS, Hermans IF, Cerundolo V (2017) Activation of human mucosal-associated invariant T cells induces CD40L-dependent maturation of monocyte-derived and primary dendritic cells. J Immunol 199:2631–2638. https://doi.org/10.4049/jimmunol.1700615
Salio M, Valitutti S, Lanzavecchia A (1997) Agonist-induced T cell receptor down-regulation: molecular requirements and dissociation from T cell activation. Eur J Immunol 27:1769–1773. https://doi.org/10.1002/eji.1830270726
Acknowledgments
We acknowledge the contribution of Craig Waugh and the Weatherall Institute of Molecular Medicine flow cytometry facility for cell sorting of MAIT cells.
This work was supported by the U.K. Medical Research Council (Medical Research Council Human Immunology Unit) and Cancer Research UK (Programme Grant C399/A2291 to V.C.).
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Salio, M., Cerundolo, V. (2020). Interactions Between MAIT Cells and Dendritic Cells. In: Kaipe, H., Magalhaes, I. (eds) MAIT Cells. Methods in Molecular Biology, vol 2098. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0207-2_8
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DOI: https://doi.org/10.1007/978-1-0716-0207-2_8
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