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HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR)

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Animal Cell Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2095))

Abstract

The platforms for bioprocess development have been developed in parallel to the needs of the manufacturing industry of biopharmaceuticals, aiming to ensure the quality and safety of their products. In this sense, Quality by Design (QbD) and Process Analytical Technology (PAT) have become the pillars for quality control and quality assurance.

A new combination of Shake Flask Reader (SFR) and Respiration Activity Monitoring System for online determination of OTR and CTR (RAMOS) allows online monitoring of main culture parameters needed for bioprocess development (pH, pO2, OTR, CTR, and QR) as presented below. Eventually, a case study of the application of the combination of SFR-RAMOS system is presented. The case study discloses the optimization of HEK293 cells cultures through the manipulation of their metabolic behavior.

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Author information

Authors and Affiliations

  1. Adolf Kühner AG, Birsfelden, Switzerland

    Tibor Anderlei

  2. Kuhner Shaker Inc., San Carlos, CA, USA

    Michael V. Keebler

  3. Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Cerdanyola del Vallès, Spain

    Jordi Joan Cairó & Martí Lecina

  4. Bioengineering Department, IQS, Universitat Ramon Llull, Barcelona, Spain

    Martí Lecina

Authors
  1. Tibor Anderlei
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  2. Michael V. Keebler
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  3. Jordi Joan Cairó
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  4. Martí Lecina
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Corresponding author

Correspondence to Martí Lecina .

Editor information

Editors and Affiliations

  1. Institute of Bioprocess and Biosystems Engineering, Hamburg University of Technology (TUHH), Hamburg, Germany

    Ralf Pörtner

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Anderlei, T., Keebler, M.V., Cairó, J.J., Lecina, M. (2020). HEK293 Cell-Based Bioprocess Development at Bench Scale by Means of Online Monitoring in Shake Flasks (RAMOS and SFR). In: Pörtner, R. (eds) Animal Cell Biotechnology. Methods in Molecular Biology, vol 2095. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0191-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0191-4_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0190-7

  • Online ISBN: 978-1-0716-0191-4

  • eBook Packages: Springer Protocols

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