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Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells

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Retinal Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2092))

Abstract

The stereotypic dendritic morphology is one of the landmark characteristics for classifying retinal ganglion cell (RGC) subtypes. These unique dendritic morphologies and their corresponding stratification level in the inner plexiform layer are indicators of their physiological function and presynaptic connection with other neurons. Mis-patterned dendritic morphologies underlie many neurological disease conditions. To streamline the morphological analysis of RGCs, here, we describe a simple protocol using Cre-/lox-dependent genetically directed sparse labeling strategy on flat-mounted retinas to inspect dendritic morphology of specific RGC subtypes.

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Acknowledgments

We acknowledge grant support from the National Institutes of Health-National Eye Institute (EY024376 to C.-A.M.), National Eye Institute Vision Core Grant (P30EY028102 to UTHealth), and the Hermann Eye Fund (UTHealth). We acknowledge Dr. Tudor Badea (National Eye Institute) for RosaiAP and Pou4f1CKOAP mouse lines, Ping Pan (UTHealth) for technical assistance, and Dr. Kimberly Mankiewicz (UTHealth) for manuscript proofreading and editing.

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Authors and Affiliations

  1. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Leila Jamal, Takae Kiyama & Chai-An Mao

  2. Biological Sciences (Neurobiology, Physiology, and Behavior), College of Liberal Arts and Sciences at Arizona State University, Tempe, AZ, USA

    Leila Jamal

Authors
  1. Leila Jamal
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  2. Takae Kiyama
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  3. Chai-An Mao
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Corresponding author

Correspondence to Chai-An Mao .

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Editors and Affiliations

  1. Ruiz Department of Ophthalmology and Visual Science, McGovern Medical School at The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Chai-An Mao

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Jamal, L., Kiyama, T., Mao, CA. (2020). Genetically Directed Sparse Labeling System for Anatomical Studies of Retinal Ganglion Cells. In: Mao, CA. (eds) Retinal Development. Methods in Molecular Biology, vol 2092. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0175-4_13

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  • DOI: https://doi.org/10.1007/978-1-0716-0175-4_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0174-7

  • Online ISBN: 978-1-0716-0175-4

  • eBook Packages: Springer Protocols

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