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Metabolic Flux Analysis in Eukaryotic Cells pp 93–118Cite as

Analyzing the Metabolism of Metastases in Mice

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2088))

Abstract

Metastasis formation is the leading cause of death in cancer patients. It has recently emerged that cancer cells adapt their metabolism to successfully transition through the metastatic cascade. Consequently, measuring and analyzing the in vivo metabolism of metastases has the potential to reveal novel treatment strategies to prevent metastasis formation. Here, we describe two different metastasis mouse models and how their metabolism can be analyzed with metabolomics and 13C tracer analysis.

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Author information

Author notes

  1. Patricia Altea-Manzano, Dorien Broekaert, and João A. G. Duarte contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, VIB, Leuven, Belgium

    Patricia Altea-Manzano, Dorien Broekaert, João A. G. Duarte, Juan Fernández-García, Mélanie Planque & Sarah-Maria Fendt

  2. Laboratory of Cellular Metabolism and Metabolic Regulation, Department of Oncology, KU Leuven and Leuven Cancer Institute (LKI), Leuven, Belgium

    Patricia Altea-Manzano, Dorien Broekaert, João A. G. Duarte, Juan Fernández-García, Mélanie Planque & Sarah-Maria Fendt

Authors
  1. Patricia Altea-Manzano
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  2. Dorien Broekaert
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  3. João A. G. Duarte
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  4. Juan Fernández-García
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  5. Mélanie Planque
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  6. Sarah-Maria Fendt
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Corresponding author

Correspondence to Sarah-Maria Fendt .

Editor information

Editors and Affiliations

  1. Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA

    Deepak Nagrath

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Altea-Manzano, P., Broekaert, D., Duarte, J.A.G., Fernández-García, J., Planque, M., Fendt, SM. (2020). Analyzing the Metabolism of Metastases in Mice. In: Nagrath, D. (eds) Metabolic Flux Analysis in Eukaryotic Cells. Methods in Molecular Biology, vol 2088. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0159-4_6

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  • DOI: https://doi.org/10.1007/978-1-0716-0159-4_6

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0158-7

  • Online ISBN: 978-1-0716-0159-4

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