Thermodynamic Approaches in Flux Analysis

  • Sabine PeresEmail author
  • Vincent Fromion
Part of the Methods in Molecular Biology book series (MIMB, volume 2088)


Networks of reactions inside the cell are constrained by the laws of mass and energy balance. Constrained-based modelling (CBM) is the most used method to describe the mass balance of metabolic network. The main key concepts in CBM are stoichiometric analysis such as elementary flux mode analysis or flux balance analysis. Some of these methods have focused on adding thermodynamics constraints to eliminate non-physical fluxes or inconsistencies in the metabolic system. Here, we review the main different approaches and how they tackle the different class of problems.

Key words

Thermodynamic in constraint-based modelling Metabolic networks Gibbs free energy Equilibrium constant of reactions 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.LRI, Université Paris-Sud, CNRSUniversité Paris-SaclayOrsayFrance
  2. 2.INRA, UR1404, MaIAGEUniversité Paris-SaclayJouy-en-JosasFrance

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