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Neutrophil pp 301–324Cite as

Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2087))

Abstract

Activation of professional phagocytes, potent microbial killers of our innate immune system, is associated with an increased cellular consumption of molecular oxygen (O2). The O2 molecules consumed are reduced by electrons delivered by a membrane localized NADPH-oxidase that initially generate one- and two electron reduced superoxide anions (O2 −) and hydrogen peroxide (H2O2), respectively. These oxidants can then be processed into other highly reactive oxygen species (ROS) that can kill microbes, but that may also cause tissue destruction and drive other immune cells into apoptosis. The development of basic techniques to measure and quantify ROS generation by phagocytes is of great importance, and a large number of methods have been used for this purpose. A selection of methods (including chemiluminescence amplified by luminol or isoluminol, absorbance change following reduction of cytochrome c, and fluorescence increase upon oxidation of PHPA) are described in detail in this chapter with special emphasis on how to distinguish between ROS that are released extracellularly, and those that are retained within intracellular organelles. These techniques can be valuable tools in research spanning from basic phagocyte biology to diagnosis of diseases linked to the NADPH-oxidase and more clinically oriented research on innate immune mechanisms and inflammation.

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Acknowledgments

This work was supported by the Swedish Research Council, the Swedish Society for Medical Research, the IngaBritt and Arne Lundberg Research Foundation, the Swedish state under the LUA-ALF and TUA agreements, the Swedish Heart- and Lung Foundation, and the King Gustaf V Memorial Foundation. We thank Maria Hjulström and Hülya Çevik-Aras for performing chemiluminescence determinations with the National diagnostic kit and L-012, respectively.

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Authors and Affiliations

  1. Department of Rheumatology and Inflammation Research, Institute of Medicine, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

    Claes Dahlgren & Martina Sundqvist

  2. Department of Oral Microbiology and Immunology, Institute of Odontology, Sahlgrenska Academy at University of Gothenburg, Gothenburg, Sweden

    Halla Björnsdottir, Karin Christenson & Johan Bylund

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  1. Claes Dahlgren
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  2. Halla Björnsdottir
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  3. Martina Sundqvist
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  4. Karin Christenson
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  5. Johan Bylund
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Correspondence to Claes Dahlgren .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA

    Mark T. Quinn

  2. Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Frank R. DeLeo

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Dahlgren, C., Björnsdottir, H., Sundqvist, M., Christenson, K., Bylund, J. (2020). Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes. In: Quinn, M., DeLeo, F. (eds) Neutrophil. Methods in Molecular Biology, vol 2087. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0154-9_22

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  • DOI: https://doi.org/10.1007/978-1-0716-0154-9_22

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