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Genome-Scale Transcript Analyses of Human Neutrophils

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Neutrophil

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2087))

Abstract

Transcriptome analyses of unicellular and multicellular organisms have changed fundamental understanding of biological and pathological processes across multiple scientific disciplines. Over the past 15 years, studies of polymorphonuclear leukocyte (PMN or neutrophil) gene expression on a global scale have provided new insight into the molecular processes that promote resolution of infections in humans. Herein we present methods to analyze gene expression in human neutrophils using Affymetrix oligonucleotide microarrays and next-generation sequencing. Notably, the procedures utilize commercially available reagents and materials and thus represent a standardized approach for evaluating PMN transcript levels.

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Acknowledgments

This work was supported by the Intramural Program of the National Institutes of Allergy and Infectious Diseases, NIH.

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Authors and Affiliations

  1. Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Scott D. Kobayashi & Adeline R. Porter

  2. Genomics Unit, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Sarah L. Anzick & Dan E. Sturdevant

  3. Laboratory of Bacteriology, Rocky Mountain Laboratories, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Frank R. DeLeo

Authors
  1. Scott D. Kobayashi
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  2. Adeline R. Porter
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  3. Sarah L. Anzick
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  4. Dan E. Sturdevant
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  5. Frank R. DeLeo
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Corresponding author

Correspondence to Frank R. DeLeo .

Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA

    Mark T. Quinn

  2. Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Frank R. DeLeo

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Kobayashi, S.D., Porter, A.R., Anzick, S.L., Sturdevant, D.E., DeLeo, F.R. (2020). Genome-Scale Transcript Analyses of Human Neutrophils. In: Quinn, M., DeLeo, F. (eds) Neutrophil. Methods in Molecular Biology, vol 2087. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0154-9_21

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  • DOI: https://doi.org/10.1007/978-1-0716-0154-9_21

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0153-2

  • Online ISBN: 978-1-0716-0154-9

  • eBook Packages: Springer Protocols

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