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Labeling Acidic Compartments of Neutrophils with Cresyl Violet

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Neutrophil

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2087))

Abstract

We introduce the acidotropic marker cresyl violet to stain acidic granules in live neutrophils. Cresyl violet is less phototoxic, more photostable, and more cost-effective than other commercially available acidotropic markers. Additionally, it does not photoconvert to fluorescent species of a different color, a limitation of other commonly used acidotropic markers. Staining can be readily detected by fluorescence microscopy or by flow cytometry, and can be used as a readout of degranulation in activated neutrophils.

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Acknowledgments

P.P.O is supported by a Studentship from the Canadian Institutes of Health Research (CIHR). Work in the authors’ laboratory is supported by grant FDN-143202 from CIHR to S.G.

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Authors and Affiliations

  1. Program in Cell Biology, Hospital for Sick Children, Toronto, ON, Canada

    Philip P. Ostrowski, Ziv Roth & Sergio Grinstein

  2. Department of Biochemistry, University of Toronto, Toronto, ON, Canada

    Philip P. Ostrowski & Sergio Grinstein

Authors
  1. Philip P. Ostrowski
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  2. Ziv Roth
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  3. Sergio Grinstein
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Corresponding author

Correspondence to Sergio Grinstein .

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Editors and Affiliations

  1. Department of Microbiology and Immunology, Montana State University, Bozeman, MT, USA

    Mark T. Quinn

  2. Laboratory of Bacteriology, Rocky Mountain Laboratories Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA

    Frank R. DeLeo

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Ostrowski, P.P., Roth, Z., Grinstein, S. (2020). Labeling Acidic Compartments of Neutrophils with Cresyl Violet. In: Quinn, M., DeLeo, F. (eds) Neutrophil. Methods in Molecular Biology, vol 2087. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0154-9_15

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  • DOI: https://doi.org/10.1007/978-1-0716-0154-9_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0153-2

  • Online ISBN: 978-1-0716-0154-9

  • eBook Packages: Springer Protocols

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