Abstract
This chapter describes three methods for measuring the bactericidal activity of neutrophils. All utilize colony counting techniques to quantify viable bacteria. A simple “one-step” protocol provides a composite measure of phagocytosis and killing, while a “two-step” protocol that separates extracellular and intracellular bacteria allows calculation of rate constants for both of these processes. We also present a method for selectively monitoring the long-term survival of bacteria within the phagosome. This may have application in identifying resistant strains and searching for compounds that sensitize pathogens to destruction.
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Magon, N.J., Parker, H.A., Ashby, L.V., Springer, R.J., Hampton, M.B. (2020). Analysis of Neutrophil Bactericidal Activity. In: Quinn, M., DeLeo, F. (eds) Neutrophil. Methods in Molecular Biology, vol 2087. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0154-9_12
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DOI: https://doi.org/10.1007/978-1-0716-0154-9_12
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-0153-2
Online ISBN: 978-1-0716-0154-9
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