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Crystallization of a Complex Between MYC and Jas Motif

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Jasmonate in Plant Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2085))

Abstract

In the jasmonate signaling pathway, a region of 17 amino acids within the Jas motif of JAZ proteins and a conserved region within the N-terminus of MYC proteins are sufficient for JAZ–MYC interactions. Crystal structures of Jas–MYC complexes have revealed the structural basis of this important interaction. Here, we describe methods of cloning, expression, and purification of MYC N-terminal proteins and their co-crystallization with Jas motif peptides.

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Acknowledgments

This work was supported by The Fundamental Research Funds for the Central Universities KYZ201817, by Henry Fok Young Teachers fund 160022, by Asia-hub grand Asia Hub on WEF and Agriculture grand 2017-AH-03, and by National Natural Science Foundation of China grand 31871996.

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Authors and Affiliations

  1. College of Plant Protection, Nanjing Agricultural University, Nanjing, China

    Feng Zhang

  2. Laboratory of Structural Biology and Biochemistry, Van Andel Research Institute, Grand Rapids, MI, USA

    Karsten Melcher

  3. Department of Energy Plant Research Laboratory, East Lansing, MI, USA

    Sheng Yang He

Authors
  1. Feng Zhang
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  2. Sheng Yang He
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  3. Karsten Melcher
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Corresponding author

Correspondence to Feng Zhang .

Editor information

Editors and Affiliations

  1. UMR DIADE - IRD, Montpellier, France

    Antony Champion

  2. UMR DIADE - IRD, Montpellier, France

    Laurent Laplaze

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Zhang, F., He, S.Y., Melcher, K. (2020). Crystallization of a Complex Between MYC and Jas Motif. In: Champion, A., Laplaze, L. (eds) Jasmonate in Plant Biology. Methods in Molecular Biology, vol 2085. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0142-6_10

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  • DOI: https://doi.org/10.1007/978-1-0716-0142-6_10

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0141-9

  • Online ISBN: 978-1-0716-0142-6

  • eBook Packages: Springer Protocols

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